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The physical training was defined as self-reported mean hours of exercise training each week during the past five years, and consisted mainly of high intensity resistance training. The Doped group consists of a mixture of bodybuilders, strongmen competitors and weightlifters whereas the Clean group consists of weightlifters only. The mode of resistance training differs slightly between the two groups; the Doped group used both 1’4 repetitions/set and 8’12 repetitions/set, while the Clean group used mainly 1’4 repetitions/set. We have to emphasize that this is the only ethically feasible approach to study long term effects of AAS abuse on athletes. Muscle strength and body composition. Subjects performed static squats at a 105′ knee angle in a custom-made Smith squat machine and ground reaction forces were recorded by AMTI force plates (464 ? 508 mm, Advanced Mechanical Technology Incorporated, Massachusetts, USA). Forces were recorded in x, y and z directions at 100 Hz using the Qualisys Track Manager (QTM) software (Qualisys AB, Gothenburg, Sweden). Maximal force (one single recording), Mean of the highest 50 recordings and Mean of 0. Personal records (PR) from competition (without tight suits) or equivalent (not all participants had competed in all disciplines) for Bench press, Squat lift and Deadlift were also used for comparisons. Blood sample of 10 ml was collected from all subjects the same time in the morning after overnight fasting by venipuncture from the cubital vein. Because we could not perform regular doping tests on the subjects and the Doped subjects were not on a ‘cycle’, indirect indicator of blood hormone level was used to prove/disprove the use of AAS. Skeletal muscle biopsies were obtained from the vastus lateralis muscle using standard needle or forceps biopsy technique [18], [19]. The biopsies were taken from the middle part of the muscle, mounted in OCT compound (Tissue Tek, Miles laboratories, Naperville, IL, USA) and then frozen in liquid propane chilled with liquid nitrogen and stored at ?80’C until further processing. Due to technical problems, one biopsy from the Clean group was discarded, leaving 6 biopsies in the Clean group for analysis. Serial muscle cross-sections were cut at ?20’C by using a Reichert Jung cryostat (Leica, Nussloch, Germany). Eight ?m thick sections were stained with haematoxylin-eosin and a modified Gomori trichrome staining for basic histopathology including detection of degenerative processes and inflammation [20]. Five ?m thick transverse sections were processed for IHC with different and previously characterized antibodies. For fiber phenotype type classification, serial sections were stained with monoclonal antibodies (mAbs) against different MyHC isoforms: A4. All antibodies were obtained from the Developmental Studies Hybridoma Bank, developed under the auspices of the National Institute of Child Health and maintained by the University of Biological Sciences, Iowa City, Iowa, USA. For fiber size measurement and capillary visualization, mAb 5H2 against laminin ?2 chain in muscle fiber basement membrane (Nova Castra Lab, Newcastle, UK) and mAb 4C7 against laminin ?5 in capillary basement membrane (Chemicon, Temecula, Calif. The IHC staining process is the same as described earlier [22]. Visualization of bound primary antibody was performed using indirect unconjugated immunoperoxidase technique and/or indirect immunofluorescence technique with affinity-purified Abs specifically prepared for multiple labelling and conjugated with flurochromes with different emission spectra, fluorescein (FITC), Rhodamine red-X (RRX) and Cyanine 5 (Cy5), gp oral tren 250 mcg oral steroids $44.00 oral tren. Nuclei were identified with 4?, 6-diamidino-2 phenylindole (DAPI) provided in the mounting media (Vectashield, Vector laboratories Inc, Burlin-game CA 94010 USA). All antibodies were diluted in 0. Control sections were treated similarly except that the primary antibodies were exchanged with non-immune serum. undefined Oral tren, manufacturer: dragon pharma category: oral steroids substance: methyltrienolone package: 100 tabs (250 mcg. Trenbolone is the most powerful overall steroid in use by bodybuilders today. Tren, also known as trenbolone is among the favorite steroids in the bodybuilding world. It is one of the most powerful compounds as well. — see a doctor if you have any concerns about your steroid treatment. Can i take other medicines when i am taking steroids? potentially, many. Oral tren contains methyltrienolone 250 mcg per tablet. An interesting fact: the only anabolic steroid that is not detectable at all. — gp oral tren 250 mcg geneza pharmaceuticals $44. 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